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Protein expression and purification GST-FIP200-MBP, FIP200(N-640)-MBP, FIP200(636-C)-MBP and full-length ULK1 complex protein samples used for NSEM, HDX-MS, fluorescent pulldown assays, ADP-Glo kinase assays and liposome sedimentation assays were expressed in HEK293-GnTI suspension cells by using the polyethylenimine (Polysciences) transfection system